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KMID : 0900919980220010101
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Korean journal of Animal Reproduction
1998 Volume.22 No. 1 p.101 ~ p.104
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A Study on Culture Environments of In Vitro Matured/In Vitro Fertilized Bovine Embryos II. Effect of Growth Factors on In Vitro Development of Bovine Embryos
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Lee Myung -Shik
Kim Chang-Keun
Chung Yung-Chai
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Abstract
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This study examined the effects of growth factors in TCM199 on bovine 1-cell embryos development in vitro. After 6 day to 11 day in culture, 15.8%(19/120), 15.3%(20/130), 21.8%(35/160), 27.0%(56/207), 26.3%(53/201) and 30.7%(40/130) of the 1-cell embryos developed into expanding blastocysts in su, pp.ementing TCM199 with control, insulin, IGF-I, IGF-II, FGF and EGF, respectively. Hatching rate of 1-cell embryos in su, pp.ementing TCM199 with FGF, EGF and IGF-II were 21.4%(53/247), 20.3%(42/206) and 16.8%(41/243), respectively. The beneficial effect of growth factors on embryo development in vitro could be duplicated. These data indicate that the presence of FGF, EGF or IGF-II in the culture medium is beneficial for embryo development in vitro and accelerate cell differentiation.
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KEYWORD
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Embryo, In vitro development, Growth factor, Hatched blastocyst
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